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Image Search Results
Journal: Biotechnology Journal
Article Title: Optimizing Lentiviral Vector Production: Insights Into PiggyBac Transposase and Concatemeric Array Strategies
doi: 10.1002/biot.70135
Figure Lengend Snippet: DNA constructs and transfection optimization . Schematic depiction of a concatemeric‐array (A) and a transposon vector (B). Dox P = doxycycline regulated promotor, LVV Rep. = Sequences necessary for LVV packaging, P = Promotor, GOI = Gene of interest, […. .] = placeholder for multiple copies of GOI, BleR = bleomycin resistance marker, AmpR = ampicillin resistance marker, ITR = inverted terminal repeat. Transfection optimization overview of different protocols and the effects on transfection efficiency (C) and cell viability (D) (fully colored bars = shaken cultivation, striped bar = static cultivation).
Article Snippet: For transfection of GPRTG cells in 24‐well plates, two different systems were used: the Neon transfection device (Thermo Fisher Scientific) with 10 and 100 μL tips and the
Techniques: Construct, Transfection, Plasmid Preparation, Marker